A disconnect can often be observed between on-target biochemical compound effect and potency in relevant cellular assays. Determination of compound cell accumulation is a means to probe this discrepancy.
At Domainex, our assay biology and analytical chemistry experts have established cell accumulation assays that deliver a concentration profile over time, revealing important information explaining compound behaviour, off target effects and non-penetrating compounds. These can be accompanied by measurements of intracellular compound binding, which can show why compounds with similar cell penetration abilities have different efficacies. This information helps our medicinal chemists to better understand compound properties.