To generate an X-ray crystal structure, samples are first crystalised and then an X-ray beam is used to create a diffraction pattern from which the position of each atom in the crystallized molecule is determined. Domainex has extensive expertise and the facilities in-house to undertake high-throughput screening of thousands of crystallisation conditions. Through a network of world-class synchrotron facilities across Europe, with whom we have established access agreements, including the Diamond Light Source (Oxfordshire, UK), we will obtain high-resolution datasets for your target proteins (both apo or ligand-bound).
X-ray crystallography is currently the gold-standard in terms of protein/protein-ligand structure generation and can be applied to proteins with a broad range of molecular weights. Data collection and structure refinement is more straightforward than for the complementary technique of cryogenic electron microscopy (Cryo-EM) and the resolution is typically higher.
For examples that showcase our capabilities in X-ray crystallography please see our MEK-1, CD73 and G9a case studies.
Figure 1: X-ray crystal structure of CD73 bound to adenosine 5′-(α,β-methylene)diphosphate (AMPCP) a CD73 inhibitor (dataset resolution 1.7Å). The structure overlays well with the Apo open conformation structure which was also solved.
For proteins which are not amenable to X-ray crystallography (for instance large complexes, membrane proteins or proteins which do not crystalise readily), we also offer Cryo-EM services.