Hit Discovery and High-Throughput Screening

robust assays • smart libraries • rapid screening • actionable hits

Flexible screening strategies from focused compound selections to miniaturised 1536-well high-throughput screening campaigns

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Domainex provides flexible hit discovery solutions tailored to your target, assay format and project goals. From focused compound selections and biophysical screening to miniaturised high-throughput screening in 384- and 1536-well formats, our integrated discovery teams help identify high-quality starting points for drug discovery. 

Key Capabilities

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High-throughput Screening (HTS) Workflow at Domainex

Phase 1
Phase 2
Phase 3
Phase 4
Phase 5
Assay Development

Develop robust biochemical or cell-based assays, optimised for reliable, reproducible screening.

Library Selection

Match commercial, focused or target-directed compound libraries to the biological target.

HTS Campaign

Run automated screening in 384- and 1536-well formats across thousands to hundreds of thousands of compounds.

Hit Confirmation

Validate and re-test active compounds to distinguish genuine, tractable hits from artefacts.

Hit-to-Lead

Progress confirmed hits with medicinal chemistry, structural biology and computational chemistry. 

Phase 1 — Assay Development
  • Develop robust biochemical or cell-based assays, optimised for reliable, reproducible screening
Phase 2 — Library Selection
  • Match commercial, focused or target-directed compound libraries to the biological target
Phase 3 — HTS Campaign
  • Run automated screening in 384- and 1536-well formats across thousands to hundreds of thousands of compounds.
Phase 4 — Hit Confirmation
  • Validate and re-test active compounds to distinguish genuine, tractable hits from artefacts
Phase 5 — Hit-to-Lead
  • Progress confirmed hits with medicinal chemistry, structural biology and computational chemistry

Multiple Routes to Hit Discovery

Choosing the right screening strategy

Not every project requires a million-compound screen. We help clients select the most appropriate approach, from focused screening and biophysical methods through to large-scale high-throughput screening campaigns.

 

Targeted and Focused Screening

Large high-throughput screening campaigns are not always the most efficient route to identifying quality hits. Domainex can support screening using commercially sourced, focused, diversity or client-owned compound collections, helping tailor each campaign to the target, assay format and project objectives.

Miniaturised High-Throughput Screening

Miniaturised high-throughput screening remains a powerful approach for identifying novel starting points for drug discovery, particularly where robust biochemical or cell-based assays can be transferred into 384- or 1536-well formats.

Domainex supports:

Integrated Hit Follow-Up

Hits identified through screening can be rapidly progressed using Domainex's medicinal chemistry, computational chemistry, protein science and structural biology capabilities.

Why Partner with Domainex?

Domainex combines robust assay development, precision low-volume dispensing, flexible screening strategies and integrated discovery expertise to help clients move efficiently from assay development to actionable hit series.

Looking for the right screening strategy?

Whether you require a large-scale high-throughput screening campaign, focused library screening, Spectral Shift screening or a fully integrated hit discovery programme, Domainex can help identify high-quality starting points for your drug discovery project.

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