If you need well-characterised, high-quality protein for your drug discovery programmes or for other purposes, then Domainex’s team of highly experienced protein scientists are at your disposal.
We are able to produce and purify the proteins you want in multi-milligram quantities, and our expertise in protein characterisation means that you can have confidence that we will deliver you high quality protein that has passed our rigorous quality control process.
Protein Science Services
We can offer you a complete protein science solution, including:
- Construct design
- Expression system optimisation using E. coli, insect cells, or mammalian cells
- Protein production
- Purification by affinity, ion-exchange and size-exclusion chromatography
- Protein characterisation
Combinatorial Domain Hunting
Often our scientists will be able to use literature-informed or bioinformatics-based approaches for expression construct design. But if you already know that these methods have been unsuccessful, or you think they will not work on your protein, our unique proprietary technology Combinatorial Domain Hunting (CDH) allows us to quickly identify soluble, highly expressible protein constructs for the most problematic drug target proteins.
The high quality of the proteins that we produce means that you can use them to support a number of key processes in drug discovery; and naturally our protein production capability pipelines smoothly into other Domainex services such as:
- X-ray crystallography leading to structure-based drug design
- Assay development
- Biochemical and Biophysical screening for Hit identification
- Fragment screening using our FragmentBuilder approach
An exciting recent development at Domainex is our PoLiPa technology for making pure and stable preparations of solubilised membrane proteins such as GPCRs. The PoLiPa approach seems set to revolutionise membrane protein drug discovery, making these otherwise difficult targets accessible to structural biology, biophysical assays, and other screening technologies.