Protein Expression and Purification Services

High quality proteins to accelerate drug discovery

If you need well-characterised, high-quality protein for your drug discovery programmes or for other purposes, then Domainex’s team of highly experienced protein scientists are at your disposal.

We are able to produce and purify the proteins you want in multi-milligram quantities, and our expertise in protein characterisation means that you can have confidence that we will deliver you high quality protein that has passed our rigorous quality control process.

Protein Science Services

We can offer you a complete protein science solution, including:

Construct design
Cloning
Protein expression services and expression system optimisation using E. coli, insect cells, or mammalian cells
Protein production services
Purification by affinity, ion-exchange and size-exclusion chromatography
Protein characterisation

Combinatorial Domain Hunting

Often our scientists will be able to use literature-informed or bioinformatics-based approaches for expression construct design. But if you already know that these methods have been unsuccessful, or you think they will not work on your protein, our unique proprietary technology Combinatorial Domain Hunting (CDH) allows us to quickly identify soluble, highly expressible protein constructs for the most problematic drug target proteins.

Membrane Proteins

Our protein scientists have optimised a screening platform to identify the best approach to purify your membrane protein, whether that is a detergent-based or detergent-free strategy.

Domainex's PoLiPa technology generates pure and stable preparations of solubilised membrane proteins such as GPCRs. The PoLiPa approach seems set to revolutionise membrane protein drug discovery, making these otherwise difficult targets accessible to structural biology, biophysical assays, and other screening technologies.

eProtein Discovery Instrument

Domainex is the only contract research organisation (CRO) in the world with an eProtein Discovery^TM instrument from Nuclera. The system is designed to speed up protein expression and purification optimization and significantly reduces the time and cost of these processes by automating construct screening and protein scale-up. Our team of highly experienced protein scientists have undertaken extensive training, enabling them to apply the instrument to your drug discovery programmes. Find out more here.

Protein Characterisation

The high quality of the proteins that we produce means that you can use them to support a number of key processes in drug discovery; and naturally our protein production capability pipelines smoothly into other Domainex services such as:

X-ray crystallography leading to structure-based drug design
Assay development
Biochemical and Biophysical screening for Hit identification
Fragment screening using our FragmentBuilder approach