We deploy state-of-the art biophysical methods to provide exquisite insight on interactions of compounds with biological targets.
Recent advances in biophysical and structural techniques mean that it is now possible to use several label-free approaches to gain crucial insights into ligand-target engagement by understanding the structural basis of drug binding, as well as measuring affinities (dissociation constants), kinetics (kon and koff) and energetics (enthalpic and entropic contributions to drug binding). Domainex is one of the leading experts in generation and interpretation of this data, and we use this knowledge on a routine basis to support both the Hit Identification and Lead Characterisation phases of your drug research projects.
We are able to deploy a suite of high-end technology platforms on your programmes:
- NanoTemper thermo-optical instruments, using read-outs based on MicroScale Thermophoresis (MST) and Temperature Related Intensity Change (TRIC)
- X-Ray Crystallography
- Differential Scanning Fluorimetry (DSF)
- Nano Differential Scanning Fluorimetry (NanoDSF)
- NMR STD & WaterLOGSY
- Surface Plasmon Resonance (SPR) and Grating-Coupled Interferometry (GCI)
- Isothermal Titration Calorimetry (ITC)
- Dynamic Light Scattering Services (DLS)
We are adept at deploying these techniques to inform your research project in an efficient and insightful manner. In addition to this, the techniques of MicroScale Thermophoresis (MST) and Temperature Related Intensity Change (TRIC) are much newer and Domainex has developed unique know-how that has made us widely recognised as a world-leader in its use.
As an MST service provider, Domainex employs state-of-the-art NanoTemper biophysical instruments (utilising the techniques of MicroScale Thermophoresis and Temperature Related Intensity Change). These techniques are at the core of our fragment screening platform FragmentBuilder. We also use these instruments for screening and characterising compounds arising from our LeadBuilder virtual screens and subsequently through lead optimisation campaigns.
Find out more about the range of instruments we use and the advantages of these techniques here.